ABSTRACT
IgA nephropathy(IgAN)is the most common primary glomerulonephritis in children and adolescents.It is an important cause of chronic kidney disease and end stage renal disease.The pathogenesis of IgAN has not been fully elucidated and it is thought to be associated with a multi-hit hypothesis, namely, increased levels of galactose-deficient IgA1(Gd-IgA1)(Hit 1); production of auto-antibodies directed against Gd-IgA1(Hit 2); formation of Gd-IgA1-containing immune complexes(Hit 3); the deposition of immune complexes in the glomerular mesangium resulting in glomerular injury(Hit 4). Gd-IgA1 is regarded as the initiator of the pathogenesis of IgAN.Core 1, β1, 3-galactosyltransferase(C1GALT1)is a key enzyme in the process of O-glycosylation of IgA.The reduction in the activity and/or gene expression of C1GALT1 is closely related to Gd-IgA1.This review will illustrate the role of C1GALT1 in the pathogenesis, diagnosis, treatment and prognosis of IgAN to provide molecular strategies for the clinical practice.
ABSTRACT
Objective To explore the clinical value of dynamic electrocardiogram ( DCG) in the diagnosis of coronary artery damage (CAD) in children with Kawasaki disease (KD).Methods A total of 125 cases of children with KD admitted to hospital in recent years was analyzed , without CAD as the control group (83cases), and others as the experimental group (42cases).All cases were undertaken DCG and electrocardiogram (ECG).The results of heart rate, heart Rhythm, and voltage changes were compared be-tween two groups .Results The abnormal rate of total ECG and DCG in the control group was significantly lower than the experimental group ( P 0.05 ) , but DCG abnormal rate of experimental group was significantly lower than ECG (χ2 =15.30, P 0.05 ) .Conclusions DCG test provides scientific parameter in the diagnosis of CAD in children with KD , and is superior to normal ECG , but the value of Joint is the same .
ABSTRACT
Objective To investigate angiotensin converting enzyme (ACE) gene insertion/deletion (I/D) polymorphism in patients with hypertension in the elderly and it's relation with serum ACE level among Chinese population.Methods Polymorphism of ACE gene was categorized into three genotype:two deletion alleles (genotype DD),heterozygous alleles (genotype ID),and two insertion alleles (genotype II).Polymerase chain reaction(PCR) was used to examine ACE gene polymorphism.Compared the distribution of three ACE genotypes frequencies and D allele frequency in 56 patients with hypertension in the elderly and 40 healthy subjects,whose serum ACE levels were measured.Results The frequency of D allele was higher in the hypertension group (0.61) than in healthy subjects (0.46) (P<0.05).A significant difference in serum ACE level was observed between the hypertensives and healthy (31.0±11.1) U/L and (25.1±10.0) U/L respectively,P<0.05.The highest level of serum ACE activity was in genotype DD,and the lowest level in genotype II,and intermediate in genotype ID.A significant difference in serum ACE level was observed among the genotype DD and ID,and among the genotype DD and II.Conclusion ACE gene I/D polymorphism was associated with serum ACE level.D allele might be a genetic risk factor for hypertension in the elderly.
ABSTRACT
Objective To investigate the association between the insertion/deletion(I/D) polymorphism of angiotensin converting enzyme (ACE) gene and the myocardial infarction (MI) among Chinese population. Methods Polymerase chain reaction(PCR) was applied to the examination of ACE gene polymorphism. A comparison was performed between 50 patients with MI and another 50 healthy subjects.Results The frequencies of DD genotype (0.38) and D allele (0.58) were both higher among the MI group than that among the control group (0.16 and 0.41 respectively,P